Acetylcholinesterase (AChE) inhibitors are the best available pharmacotherapy for the treatment of various\nneurological disorders such as Alzheimerâ��s disease, senile dementia, ataxia, myasthenia gravis, glaucoma and paralytic ileus.\nEllmanâ��s method is widely used in-vitro method for evaluation of AchE inhibitory. Hexane subfraction from methanolic extract of\nN. jatamansi was prepared by soxhlet extraction method. AchE inhibiton assay of hexane subfraction of N. jatamansi showed\nbetter IC50 - 36.41 �¼g/ml. Hexane subfraction was subjected to column chromatography and each fraction was evaluated for the\nAchE inhibitory activity. Amongst 12 Column fractions C9, C11 and C12 showed 61.39%, 58.08% and 62.94% AchE inhibitory\nactivity respectively. The % inhibition of AChE enzyme by above three fractions was less than the % inhibition of hexane\nfraction, which indicates that AchE inhibitory activity of hexane fraction is not due to any single compound but it is synergistic\nactivity of several compounds present in the hexane fraction.
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